FluCalc

MSS-MLE (Ma-Sandri-Sarkar Maximum Likelihood Estimator) calculator for Luria–Delbrück fluctuation analysis.

Reference

Radchenko et. al, 2017 (Methods in Molecular Biology).
DOI: 10.1007/978-1-4939-7306-4_29

Installation

1. Clone or download source code.

2. Install Python 3.8 or upper and libraries:

   • gunicorn=20.0.4
   • flask=1.1.1
   • flask-wtf=0.14.3
   • scipy=1.4.1
   • numpy=1.18.1

3. Set FLUCALC_SECRET_KEY environment variable with a secret string. You can use any random sequence for it.

4. Change dir to the root of source code.

5. Start the server for FluCalc:

    gunicorn -w 4 -b <ip>:<port> flucalc:app

   Or you can use run_server.py script as alternative way to start the server:

   ./run_server.py :

6. For shutdown server press Ctrl + C in the terminal.